The organisms can be isolate from sputum, skin lesions, CSF and pleural fluid.
It requires special media for growth and it can be recognized after 3-5 days after inoculation onto the routine media.
Spherules can be best identified with acids. And the size of the spherules might differ from 20-80 micronmeter in diameter. They are best identified under Schiff or Gomori methamine silver stains. This reaction often reults in granulomatous reactions with fibrosisand caseation in chronic lesions.
It is based on the immunoprecipitation reaction.
In general, in mixed antigen—antibody reactions, each antigen—antibody combination will form a separate line of precipitation, distinct from those of other antigen—antibody interactions.
Immunodiffusion and enzyme immunoassay:
It is used to detect the presence of IgG and IgM antibodies. A coccidioidal antigen assay is available which may sense positive in the urine and blood of patients with extrapulmonary disease and in the CSF of those with meningitis caused by C. immitis. If a patient has typical clinical manifestations of C. immitis, then the EIA IgM positive/IDCF negative result supports the presence of disease.
Beta D Glucan:
An antigen compound which is useful in the diagnosis of valley fever.
The chest x ray predicts any abnormal area in the lungs
It reveals the slice of the lung. It uses both the combination of x ray and computer aided device.
It helps to analyse the size, shape and position of any lung tumour and also it helps in the detection of enlarged lymph nodes.
It also looks for any masses in the adrenal gland, liver, brain and other organs.
CT guided needle Biopsy:
CT scan might be used to guide a biopsy needle into this area to get the tissue for lung structure.
It uses the soft tissue image of the organ. It uses the both the magnet and radiowaves.
It reveals abnormality in the lung tissue and lymph node enlargement.