Complete blood test:
The blood test might shows the decreased level of platelet counts and other immune cells. Thrombocytopenia( decrease in the platelet count) might increases the chances for bleeding tendencies.
Nucleic acid amplification tests (NAATs):
For the patient diagnosed with the dengue virus, NAAT’s are the preferred method of laboratory diagnosis:
NAATs should be performed on serum specimens collected 7 days or less after symptom onset.
Laboratory confirmation can be made from a single acute phase serum specimen obtained early ( less than 7 days after the fever onset in the illness by detecting viral genomic sequences with Rrt-pcr or non structural proteins 1 (NS1) antigen by immunoassay.
Presence of virus by Rrt-PCR or NS1 antigen in a single diagnostic specimen is considered as laboratory confirmation test.
IgM antibody testing can identify additional infections and it is an important diagnostic tool.
Later in the illness greater than 4 days after onset of the fever, IgM against dengue virus can be detected with MAC-ELISA. For patients presenting with the symptoms during the first week diagnostic test should include a test for dengue virus (Rrt-PCR or NS1) and IgM.
For patients presenting grater than 1 week after fever onset, IgM detection is most useful.
IgM in a single serum sample strongly suggests a recent dengue virus infection and it can be taken as a probable confirmatory test.
IgG antibody testing
IgG detection by ELISA in a single serum sample is not useful for diagnostic testing because it remains detectable for life after a dengue virus infection.
Detection of virus-neutralizing antibodies in combination with recent travel history to an
endemic area may be meaningful.
Immunosorbent assay- detect the virus specific IgM or IgG antibodies.
Greater than fourfold rise in titer between acute and convalescent sera and cerebo spinal
fluid containing virus specific IgG or IgM or both are the diagnostic features.
This test involves the injection of arbovirus in to the mice cell by suckling action. Later the mice are checked for the symptoms and other features.